idh2 r140q mutant (Active Motif)
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idh2 r140q mutant
Idh2 R140q Mutant, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/idh2+r140q+mutant/idh2+r140q+mutant/pm34289383-265-89-92
Average 90 stars, based on 1 article reviews
Idh2 R140q Mutant, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/idh2+r140q+mutant/idh2+r140q+mutant/pm34289383-265-89-92
Average 90 stars, based on 1 article reviews
idh2 r140q mutant - by Bioz Stars,
2026-09
90/100 stars
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Purification:Article Title: Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells. Article Snippet: .. Purified ACAT1 WT or Y407 mutant proteins were incubated with ACAT1antibody (CST) overnight at 4 C and subsequently incubated with protein G-Sepharose for 2 hours at 4 C. After three washes with TBS, purified ACAT1 WT or Y407 mutant proteins on beads were incubated with or without purified FLT3 in buffer containing 60 mM HEPES (pH 7.5), 3 mM MgCl2, 3 mM MnCl2, 3 mM Na3VO4, 1.2 mMDTT, 800 mMATP at 30 C for 90 min, washed with TBS, and then subjected to in vitro acetyltransferase assay using purified Mutagenesis:Article Title: Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells. Article Snippet: .. Purified ACAT1 WT or Y407 mutant proteins were incubated with ACAT1antibody (CST) overnight at 4 C and subsequently incubated with protein G-Sepharose for 2 hours at 4 C. After three washes with TBS, purified ACAT1 WT or Y407 mutant proteins on beads were incubated with or without purified FLT3 in buffer containing 60 mM HEPES (pH 7.5), 3 mM MgCl2, 3 mM MnCl2, 3 mM Na3VO4, 1.2 mMDTT, 800 mMATP at 30 C for 90 min, washed with TBS, and then subjected to in vitro acetyltransferase assay using purified Incubation:Article Title: Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells. Article Snippet: .. Purified ACAT1 WT or Y407 mutant proteins were incubated with ACAT1antibody (CST) overnight at 4 C and subsequently incubated with protein G-Sepharose for 2 hours at 4 C. After three washes with TBS, purified ACAT1 WT or Y407 mutant proteins on beads were incubated with or without purified FLT3 in buffer containing 60 mM HEPES (pH 7.5), 3 mM MgCl2, 3 mM MnCl2, 3 mM Na3VO4, 1.2 mMDTT, 800 mMATP at 30 C for 90 min, washed with TBS, and then subjected to in vitro acetyltransferase assay using purified In Vitro:Article Title: Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells. Article Snippet: .. Purified ACAT1 WT or Y407 mutant proteins were incubated with ACAT1antibody (CST) overnight at 4 C and subsequently incubated with protein G-Sepharose for 2 hours at 4 C. After three washes with TBS, purified ACAT1 WT or Y407 mutant proteins on beads were incubated with or without purified FLT3 in buffer containing 60 mM HEPES (pH 7.5), 3 mM MgCl2, 3 mM MnCl2, 3 mM Na3VO4, 1.2 mMDTT, 800 mMATP at 30 C for 90 min, washed with TBS, and then subjected to in vitro acetyltransferase assay using purified Western Blot:Article Title: Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells. Article Snippet: .. Purified ACAT1 WT or Y407 mutant proteins were incubated with ACAT1antibody (CST) overnight at 4 C and subsequently incubated with protein G-Sepharose for 2 hours at 4 C. After three washes with TBS, purified ACAT1 WT or Y407 mutant proteins on beads were incubated with or without purified FLT3 in buffer containing 60 mM HEPES (pH 7.5), 3 mM MgCl2, 3 mM MnCl2, 3 mM Na3VO4, 1.2 mMDTT, 800 mMATP at 30 C for 90 min, washed with TBS, and then subjected to in vitro acetyltransferase assay using purified |